Integrated microfluidic devices for combinatorial cell-based assays (2024)

Yu, Z. T. F., Kamei, K. I., Takahashi, H., Shu, C. J., Wang, X., He, G. W., Silverman, R., Radu, C. G., Witte, O. N., Lee, K. B., & Tseng, H. R. (2009). Integrated microfluidic devices for combinatorial cell-based assays. Biomedical Microdevices, 11(3), 547-555. https://doi.org/10.1007/s10544-008-9260-x

Integrated microfluidic devices for combinatorial cell-based assays. / Yu, Zeta Tak For; Kamei, Ken Ichiro; Takahashi, Hiroko et al.
In: Biomedical Microdevices, Vol. 11, No. 3, 2009, p. 547-555.

Research output: Contribution to journal › Article › peer-review

Yu, ZTF, Kamei, KI, Takahashi, H, Shu, CJ, Wang, X, He, GW, Silverman, R, Radu, CG, Witte, ON, Lee, KB & Tseng, HR 2009, 'Integrated microfluidic devices for combinatorial cell-based assays', Biomedical Microdevices, vol. 11, no. 3, pp. 547-555. https://doi.org/10.1007/s10544-008-9260-x

Yu ZTF, Kamei KI, Takahashi H, Shu CJ, Wang X, He GW et al. Integrated microfluidic devices for combinatorial cell-based assays. Biomedical Microdevices. 2009;11(3):547-555. doi: 10.1007/s10544-008-9260-x

Yu, Zeta Tak For ; Kamei, Ken Ichiro ; Takahashi, Hiroko et al. / Integrated microfluidic devices for combinatorial cell-based assays. In: Biomedical Microdevices. 2009 ; Vol. 11, No. 3. pp. 547-555.

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title = "Integrated microfluidic devices for combinatorial cell-based assays",

abstract = "The development of miniaturized cell culture platforms for performing parallel cultures and combinatorial assays is important in cell biology from the single-cell level to the system level. In this paper we developed an integrated microfluidic cell-culture platform, Cell-microChip (Cell-μChip), for parallel analyses of the effects of microenvironmental cues (i.e., culture scaffolds) on different mammalian cells and their cellular responses to external stimuli. As a model study, we demonstrated the ability of culturing and assaying several mammalian cells, such as NIH 3T3 fibroblast, B16 melanoma and HeLa cell lines, in a parallel way. For functional assays, first we tested drug-induced apoptotic responses from different cell lines. As a second functional assay, we performed {"}on-chip{"} transfection of a reporter gene encoding an enhanced green fluorescent protein (EGFP) followed by live-cell imaging of transcriptional activation of cyclooxygenase 2 (Cox-2) expression. Collectively, our Cell-μChip approach demonstrated the capability to carry out parallel operations and the potential to further integrate advanced functions and applications in the broader space of combinatorial chemistry and biology.",

keywords = "Apoptosis, Cell culture, Cell-based assay, Microfluidic devices, Transfection",

author = "Yu, {Zeta Tak For} and Kamei, {Ken Ichiro} and Hiroko Takahashi and Shu, {Chengyi Jenny} and Xiaopu Wang and He, {George Wenfu} and Robert Silverman and Radu, {Caius G.} and Witte, {Owen N.} and Lee, {Ki Bum} and Tseng, {Hsian Rong}",

year = "2009",

doi = "10.1007/s10544-008-9260-x",

language = "English (US)",

volume = "11",

pages = "547--555",

journal = "Biomedical Microdevices",

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T1 - Integrated microfluidic devices for combinatorial cell-based assays

AU - Yu, Zeta Tak For

AU - Kamei, Ken Ichiro

AU - Takahashi, Hiroko

AU - Shu, Chengyi Jenny

AU - Wang, Xiaopu

AU - He, George Wenfu

AU - Silverman, Robert

AU - Radu, Caius G.

AU - Witte, Owen N.

AU - Lee, Ki Bum

AU - Tseng, Hsian Rong

PY - 2009

Y1 - 2009

N2 - The development of miniaturized cell culture platforms for performing parallel cultures and combinatorial assays is important in cell biology from the single-cell level to the system level. In this paper we developed an integrated microfluidic cell-culture platform, Cell-microChip (Cell-μChip), for parallel analyses of the effects of microenvironmental cues (i.e., culture scaffolds) on different mammalian cells and their cellular responses to external stimuli. As a model study, we demonstrated the ability of culturing and assaying several mammalian cells, such as NIH 3T3 fibroblast, B16 melanoma and HeLa cell lines, in a parallel way. For functional assays, first we tested drug-induced apoptotic responses from different cell lines. As a second functional assay, we performed "on-chip" transfection of a reporter gene encoding an enhanced green fluorescent protein (EGFP) followed by live-cell imaging of transcriptional activation of cyclooxygenase 2 (Cox-2) expression. Collectively, our Cell-μChip approach demonstrated the capability to carry out parallel operations and the potential to further integrate advanced functions and applications in the broader space of combinatorial chemistry and biology.

AB - The development of miniaturized cell culture platforms for performing parallel cultures and combinatorial assays is important in cell biology from the single-cell level to the system level. In this paper we developed an integrated microfluidic cell-culture platform, Cell-microChip (Cell-μChip), for parallel analyses of the effects of microenvironmental cues (i.e., culture scaffolds) on different mammalian cells and their cellular responses to external stimuli. As a model study, we demonstrated the ability of culturing and assaying several mammalian cells, such as NIH 3T3 fibroblast, B16 melanoma and HeLa cell lines, in a parallel way. For functional assays, first we tested drug-induced apoptotic responses from different cell lines. As a second functional assay, we performed "on-chip" transfection of a reporter gene encoding an enhanced green fluorescent protein (EGFP) followed by live-cell imaging of transcriptional activation of cyclooxygenase 2 (Cox-2) expression. Collectively, our Cell-μChip approach demonstrated the capability to carry out parallel operations and the potential to further integrate advanced functions and applications in the broader space of combinatorial chemistry and biology.

KW - Apoptosis

KW - Cell culture

KW - Cell-based assay

KW - Microfluidic devices

KW - Transfection

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